Related Experiment Videos
The energy requirement for protein synthesis in rat brain mitochondria purified by phase partition
M Peñas1, J Sánchez-Prieto, E Martín-González
1Departamento de Bioquímica, Facultad de Farmacia, Universidad Complutense, Madrid, Spain.
Revista Espanola De Fisiologia
|March 1, 1988
Abstract:
Brain mitochondria purified by phase partition showed a higher rate of 14C-leucine incorporation into proteins with an endogenous source of ATP than with an exogenous ATP-generating system. Under the former conditions the presence of atractyloside increased the 14C-leucine incorporation into proteins. The effects of different valinomycin concentrations plus attractyloside on intramitochondrial ATP levels and 14C-leucine incorporation into proteins have been studied. The results indicate that the protein synthesis in brain mitochondria is dependent on the intramitochondrial ATP concentration.