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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

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Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
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Cell Migration01:19

Cell Migration

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Cell migration is a process by which the cells move from one location to another, playing an essential role in embryological development, repair and regeneration, immune response, and metastasis. Cells migrate in response to chemical or mechanical signals generated by specific organs or tissues. The overall mechanism includes three steps - polarization, protrusion, and release. Polarization involves the formation of a distinct cell front and rear, which determines the direction of movement.
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Cell Migration01:09

Cell Migration

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Cell migration, the process by which cells move from one location to another, is essential for the proper development and viability of organisms throughout their life. When cells are not able to migrate properly to their ordained locations, various disorders may occur. For example, disruption in cell migration causes chronic inflammatory diseases such as arthritis.
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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Related Experiment Video

Updated: Jan 3, 2026

Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes
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Differentiation of a Human Neural Stem Cell Line on Three Dimensional Cultures, Analysis of MicroRNA and Putative Target Genes

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microRNA-1 Regulates NCC Migration and Differentiation by Targeting sec63.

Dongyue Wang1, Yajuan Weng1, Shuyu Guo1

  • 1Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University, Nanjing 210019, China.

International Journal of Biological Sciences
|November 23, 2019
PubMed
Summary

MicroRNA-1 (miR-1) is crucial for craniofacial development, regulating neural crest cell migration and differentiation by targeting SEC63. Its deficiency causes jaw defects, reversible by suppressing SEC63.

Keywords:
Cranial DefectNeural Crest CellsSec63iTRAQmicroRNA

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Preparation of Small RNA Libraries for Sequencing from Early Mouse Embryos
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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Neural crest cells are critical for craniofacial development.
  • MicroRNA-1 (miR-1) plays a role in muscle development and disease.
  • The precise role of miR-1 in neural crest cell development is largely unknown.

Purpose of the Study:

  • Investigate the effects of miR-1 on neural crest cell development in craniofacial structures.
  • Elucidate the molecular mechanisms underlying miR-1's function in this process.

Main Methods:

  • Utilized zebrafish as a model organism, employing miR-1 morpholino injection to knockdown miR-1.
  • Assessed neural crest cell migration using time-lapse imaging.
  • Analyzed gene expression patterns via whole-mount in situ hybridization.
  • Identified miR-1 targets using quantitative proteomics (iTRAQ) and bioinformatics, validating SEC63 as a direct target.

Main Results:

  • miR-1 deficiency led to craniofacial defects, including reduced lower jaw and delayed pigment cell formation.
  • Neural crest cell migration and differentiation were impaired in miR-1 deficient zebrafish.
  • SEC63 was identified as a direct target of miR-1, and its suppression partially rescued the observed defects.

Conclusions:

  • miR-1 is essential for regulating neural crest cell development during craniofacial formation.
  • miR-1 exerts its function, at least partly, through the direct targeting of SEC63.
  • This study identifies a novel mechanism involving miR-1 and SEC63 in craniofacial development.