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The human secretome.

Mathias Uhlén1,2,3, Max J Karlsson4, Andreas Hober4

  • 1Department of Protein Science, Science for Life Laboratory, KTH-Royal Institute of Technology, Stockholm, Sweden. mathias.uhlen@scilifelab.se.

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Summary
This summary is machine-generated.

Human secretome proteins are vital for biology and medicine. This study reveals many secreted proteins remain intracellular or tissue-specific, not entering the blood, with detailed data in the Human Protein Atlas.

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Area of Science:

  • Proteomics
  • Human Biology
  • Cell Biology

Background:

  • Secreted proteins (secretome) are crucial for human biology.
  • They offer potential targets for diagnostics and therapies.
  • Understanding protein localization is key.

Purpose of the Study:

  • To comprehensively analyze proteins predicted to be secreted by human cells.
  • To determine the localization of these proteins within the human body, including peripheral blood.
  • To update the Human Protein Atlas with new secretome data.

Main Methods:

  • Bioinformatic prediction of secreted proteins.
  • Analysis of protein localization data (intracellular, tissue-specific, secreted).
  • Integration of mass spectrometry and immunoassay data for blood proteins.

Main Results:

  • A significant portion of predicted secretome proteins are retained intracellularly.
  • Many others are localized to specific tissues and not secreted into the blood.
  • The Human Protein Atlas (v19) now includes detailed secretome annotations, expression, and localization data.

Conclusions:

  • The human secretome is more complex than previously thought, with extensive intracellular and tissue-specific localization.
  • This provides a valuable resource for understanding human biology and identifying therapeutic targets.
  • Updated Human Protein Atlas enhances open-access knowledge of the human secretome.