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miR-122 direct detection in human serum by time-gated fluorescence imaging

Emilio Garcia-Fernandez1, M Carmen Gonzalez-Garcia1, Salvatore Pernagallo2

  • 1Departamento de Fisicoquimica. Unidad de Excelencia de Química aplicada a Biomedicina y Medioambiente, Facultad de Farmacia, Universidad de Granada, Campus de Cartuja s/n, 18071-Granada, Spain. angelort@ugr.es.

Chemical Communications (Cambridge, England)
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Immunofluorescence Microscopy01:12

Immunofluorescence Microscopy

A fluorescence microscope uses fluorescent chromophores called fluorochromes, which can absorb energy from a light source and then emit this energy as visible light. Fluorochromes include naturally fluorescent substances (such as chlorophylls) and fluorescent stains that are added to the specimen to create contrast. Dyes such as Texas red and FITC are examples of fluorochromes. Other examples include the nucleic acid dyes 4’,6’-diamidino-2-phenylindole (DAPI), and acridine orange.
The...