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Updated: Jan 2, 2026
Detection of Fluorescent Nanoparticle Interactions with Primary Immune Cell Subpopulations by Flow Cytometry
Published on: March 28, 2014
Detection of Small Subsets of CD4+ Lymphocytes with SmartFlare Nanoprobes
A P Toptygina1, R Sh Zakirov2, K S Kapitanova3
1G. N. Gabrichevsky Moscow Research Institute for Epidemiology and Microbiology, Federal Service on Surveillance for Consumer Rights Protection and Human Well-Being, Moscow, Russia. toptyginaanna@rambler.ru.
Abstract:
SmartFlare technology allows detection of mRNA in single living cells. We studied the possibility of using SmartFlare nanoprobes for detection of small subsets of CD4+ lymphocytes. It was found that SmartFlare allows detection of transcriptional master regulators of major CD4+T helper subsets in living human lymphocytes. Nanoprobes labeled with Cy5 fluorophore were better detected by flow cytometry than nanoprobes labeled with Cy3. Appropriate time of lymphocyte incubation with SmartFlare probes was 24 h.
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