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Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
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Nanofabricated versatile electrochemical sensor for Leptospira interrogans detection.
Rupak Nagraik1, Ankur Kaushal2, Shagun Gupta1
1Faculty of Applied Sciences and Biotechnology, Shoolini University, Post Box No. 9, Head Post Office, Solan, Himachal Pradesh 173212, India.
Journal of Bioscience and Bioengineering
|December 2, 2019
Summary
A novel electrochemical sensor utilizing LipL32 gene detection for Leptospira interrogans was created. This sensor demonstrates high specificity and reproducibility for early Leptospira interrogans diagnosis.
Area of Science:
- Nanotechnology
- Biosensors
- Molecular Diagnostics
Background:
- Leptospira interrogans causes leptospirosis, a significant zoonotic disease.
- Early and accurate detection of Leptospira interrogans is crucial for effective treatment and public health.
- Existing diagnostic methods can be time-consuming or lack sensitivity.
Purpose of the Study:
- To develop a highly sensitive and specific electrochemical sensor for Leptospira interrogans detection.
- To utilize gene-based detection for improved diagnostic accuracy.
- To explore the potential of nanomaterials in biosensor development.
Main Methods:
- Fabrication of a nanofabricated electrochemical sensor using carboxylated multiwalled carbon nanotubes with gold nanoparticles (c-MWCNTs/nanoAu) and graphene quantum dots (GQDs).
- Immobilization of a 5'-Amino (NH2) labeled single-stranded DNA (ssDNA) probe targeting the LipL32 gene of Leptospira interrogans.
- Electrochemical analysis using differential pulse voltammetry (DPV) with methylene blue as a redox indicator.
Main Results:
- The sensor demonstrated good linearity with complementary ssDNA concentrations from 0.37 to 12 ng/μl.
- High specificity towards Leptospira interrogans was observed.
- The sensor exhibited good reproducibility when stored at 4°C, indicating stability.
Conclusions:
- The developed LipL32 gene-based electrochemical sensor offers a promising tool for sensitive and specific detection of Leptospira interrogans.
- The use of c-MWCNTs/nanoAu and GQDs enhances sensor performance.
- This nanofabricated sensor has potential for early diagnosis of leptospirosis.
