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Updated: Jan 2, 2026
Fabrication of Electrochemical-DNA Biosensors for the Reagentless Detection of Nucleic Acids, Proteins and Small Molecules
Published on: June 1, 2011
Nanofabricated versatile electrochemical sensor for Leptospira interrogans detection
Rupak Nagraik1, Ankur Kaushal2, Shagun Gupta1
1Faculty of Applied Sciences and Biotechnology, Shoolini University, Post Box No. 9, Head Post Office, Solan, Himachal Pradesh 173212, India.
Abstract:
In this report, a LipL32 gene based nanofabricated electrochemical sensor for the detection of Leptospira interrogans has been developed using carboxylated multiwalled carbon nanotubes with gold nanoparticles (c-MWCNTs/nanoAu) electrode and graphene quantum dots (GQDs). The c-MWCNTs/nanoAu electrode was linked to GQDs using 4-aminothiophenol (ATP). The surface modifications on the electrode surface were delineated using Raman spectroscopy and field emission scanning electron microscopy (FE-SEM). 5'-Amino (NH2) labeled single stranded DNA (ssDNA) probe was immobilized on the surface of c-MWCNTs/nanoAu/ATP/GQD composite electrode. The electrochemical changes of the developed sensor after hybridization with single stranded complementary DNA of L. interrogans were analyzed by differential pulse voltammetry (DPV) using 1 mM methylene blue. The sensor showed good linearity with complementary ssDNA concentration ranging from 0.37 to 12 ng/μl. The sensor exhibited high specificity to L. interrogans and showed good reproducibility when stored at 4°C.
