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Mesenchymal Stem Cells

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...

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Updated: Jan 2, 2026

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Isolation of Mesenchymal Stem Cells from Human Alveolar Periosteum and Effects of Vitamin D on Osteogenic Activity of Periosteum-derived Cells

Published on: May 4, 2018

Ascorbic acid enhances bone parameter expression in human gingival mesenchymal stem cells.

F Diomede¹, G D Marconi¹, M Serroni¹

¹Department of Medical, Oral and Biotechnological Sciences, Chieti, Italy.

Journal of Biological Regulators and Homeostatic Agents

|December 5, 2019

Summary

This summary is machine-generated.

Ascorbic acid (AS) enhances osteogenic differentiation in human gingival mesenchymal stem cells (hGMSCs). This vitamin C treatment upregulates bone markers and promotes calcium deposits, suggesting AS as a potential therapy for bone diseases.

Keywords:

ascorbic acid human gingival mesenchymal stem cells osteogenic markers

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Area of Science:

Biomedical Engineering
Stem Cell Biology
Regenerative Medicine

Background:

Ascorbic acid (vitamin C) is crucial for collagen biosynthesis and biological processes.
Human gingival mesenchymal stem cells (hGMSCs) offer accessible stem cell source with therapeutic potential.

Purpose of the Study:

To investigate if ascorbic acid (AS) supplementation enhances osteogenic differentiation in hGMSCs.
To determine optimal AS concentrations for promoting bone formation in vitro.

Main Methods:

hGMSCs were supplemented with AS (60 and 90 μg/mL) for three weeks.
Osteogenic differentiation was assessed via Alizarin red S staining.
Gene and protein expression of osteogenic markers (COL1A1, RUNX2, BMP2/4, OPN, SPARC) were analyzed using RT-PCR, Western blotting, and CLSM.

Main Results:

AS supplementation significantly supported osteogenic differentiation in hGMSCs.
Upregulation of key osteogenic markers and de novo calcium phosphate deposition were observed.
AS treatment demonstrated a dose-dependent effect on osteogenic markers.

Conclusions:

Ascorbic acid effectively promotes osteogenic differentiation of hGMSCs.
AS holds promise as a therapeutic agent for preventing and healing bone-related diseases.
Further research into AS for bone regeneration is warranted.