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Updated: Jan 2, 2026

Phase Behavior of Charged Vesicles Under Symmetric and Asymmetric Solution Conditions Monitored with Fluorescence Microscopy
Published on: October 24, 2017
Temperature-Dependent Dynamics of Giant Vesicles Composed of Hydrolysable Lipids Having an Amide Linkage
Maito Tameyuki1, Hisato Hiranaka1, Taro Toyota2
1Department of Applied Chemistry, Faculty of Science and Technology , Keio University , 3-14-1 Hiyoshi , Kohoku-ku, Yokohama 223-8522 , Japan.
Abstract:
Various amphiphiles including surfactants and lipids have been designed and synthesized to improve and create new functionalities. In particular, the emergence of cell-like behaviors of giant vesicles (GVs) composed of synthetic lipids has drawn much attention in the development of chemical models for cells. The aim of this study was to measure temperature-dependent morphological changes of GVs induced by fragmentation and subsequent growth using hydrolysable cationic lipids having an amide linkage. Results from differential scanning calorimetry, fluorescence spectroscopy using an environment-responsive probe, and confocal Raman microscopy showed that the dynamics observed were due to changes in the vesicle membrane, including variation in the lipid composition, induced by thermal stimulation.
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