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Three Dimensional Printing Bilayer Membrane Scaffold Promotes Wound Healing
Shoubao Wang1, Yao Xiong1, Jingting Chen1
1Department of Plastic and Reconstructive Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
Frontiers in Bioengineering and Biotechnology
|December 6, 2019
Summary
A novel 3D-printed bilayer membrane scaffold effectively promotes skin regeneration by enhancing blood vessel formation and collagen deposition, offering a promising solution for wound healing applications.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Tissue Engineering
Background:
- Full-thickness skin wounds pose significant physical and economic challenges.
- Three-dimensional (3D) printing enables the creation of advanced skin constructs for wound healing.
- Current 3D skin printing for in vivo applications faces limitations, particularly regarding vascularization and cell integration.
Purpose of the Study:
- To design and evaluate a novel 3D-printed bilayer membrane (BLM) scaffold for enhanced skin regeneration.
- To mimic the epidermis and dermis using distinct material layers for optimal wound healing.
- To assess the efficacy of the BLM scaffold in promoting skin repair compared to single-layer scaffolds and untreated defects.
Main Methods:
- Fabrication of a bilayer membrane (BLM) scaffold comprising an outer poly(lactic-co-glycolic acid) (PLGA) layer and an inner alginate hydrogel layer.
- In vitro assessment of the alginate hydrogel's porous structure for cell adhesion and proliferation.
- In vivo evaluation of the BLM scaffold's performance in skin regeneration, including histopathological analysis, CD31 staining for neovascularization, and measurement of collagen and inflammatory markers (COL1a1, COL3a1, IL-1β, TNF-α).
Main Results:
- The multi-porous alginate hydrogel layer supported cell adhesion and proliferation in vitro.
- The PLGA outer layer effectively prevented bacterial invasion and maintained hydrogel moisture.
- In vivo studies demonstrated superior skin regeneration with the BLM scaffold, characterized by increased neovascularization (CD31 expression) and enhanced collagen type I and III deposition.
- Reduced expression of inflammatory markers (IL-1β, TNF-α) was observed with the BLM scaffold.
Conclusions:
- The 3D-printed BLM scaffold effectively mimics the skin's epidermal and dermal layers.
- The BLM scaffold significantly promotes skin regeneration by enhancing vascularization and collagen synthesis.
- These findings highlight the potential of 3D-printed BLM scaffolds as advanced wound dressings or skin substitutes for diverse clinical applications.
