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Systematic Modifications of a Simple Tolan: Another Category of Viscosity Sensor
Jung-Ho Hong1, Min-Sung Ko1, P Sankara Rao1
1Department of Chemistry and Chemical Engineering , Inha University , Inharo 100 , Incheon 22212 , Republic of Korea.
Abstract:
The first tolan derivative-based viscosity sensor (5d) has been synthesized, and its fluorescence intensity and lifetime increase when the viscosity of the solvent increases in methanol-glycerol mixtures. Phthalide (5d) was selected among structurally diverse tolan derivatives through systematic modifications of a simple tolan. To test 5d as a viscosity sensor, fluorescence lifetime imaging (FLIM) images of HeLa cells were obtained upon treatment with 5 μM of 5d to map the viscosity of the HeLa cells.
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