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Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

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Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
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Electrochemiluminescence methods using CdS quantum dots in aptamer-based thrombin biosensors: a comparative study.

Ibrahim Isildak1, Farzaneh Navaeipour2, Hadi Afsharan2

  • 1Department of Bioengineering, Faculty of Chemistry-Metallurgy, Yildiz Technical University, 34220, Istanbul, Turkey. isildak@yildiz.edu.tr.

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|December 8, 2019
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Summary

This study introduces three methods for detecting thrombin using cadmium sulfide nanocrystals (CdS NCs) and gold nanoparticles (AuNPs). The ratiometric method demonstrated the highest sensitivity for thrombin detection.

Keywords:
AptamerCdS NanocrystalsElectrochemiluminescenceGold nanoparticlesRatiometric biosensorThrombin

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Area of Science:

  • Nanotechnology
  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Thrombin is a key biomarker in various physiological and pathological processes.
  • Sensitive and specific detection of thrombin is crucial for early disease diagnosis.
  • Existing detection methods may lack the required sensitivity or specificity.

Purpose of the Study:

  • To investigate novel methods for sensitive thrombin detection.
  • To compare the efficacy of quenching, amplification, and ratiometric detection strategies.
  • To develop an aptasensor for thrombin detection using nanomaterials.

Main Methods:

  • Utilized cadmium sulfide nanocrystals (CdS NCs) and gold nanoparticles (AuNPs).
  • Employed three detection approaches: quenching, amplification (via FRET), and ratiometric analysis.
  • Constructed an electrochemiluminescence (ECL)-based biosensor.

Main Results:

  • The ratiometric method achieved the highest sensitivity with a limit of detection (LOD) of 500 fg/mL.
  • The amplification method showed an LOD of 6.5 pg/mL.
  • The quenching method had an LOD of 92 pg/mL, indicating lower utility.

Conclusions:

  • The ratiometric method offers superior sensitivity for thrombin detection compared to quenching and amplification methods.
  • The developed aptasensor platform shows promise for sensitive and selective thrombin quantification.
  • Nanomaterial-based approaches provide effective strategies for biosensing applications.