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Updated: Jan 1, 2026
In Vitro Selection of Aptamers to Differentiate Infectious from Non-Infectious Viruses
Published on: September 7, 2022
Rapid isolation of bacteria-specific aptamers with a non-SELEX-based method
Hye Ri Kim1, Min Yong Song2, Byoung Chan Kim1
1Center for Environment, Health and Welfare Research, Korea Institute of Science and Technology (KIST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Republic of Korea; Division of Energy and Environment Technology, KIST School, University of Science and Technology (UST), Hwarangno 14-gil 5, Seongbuk-gu, Seoul, 02792, Republic of Korea.
This study introduces a faster method for isolating bacteria-specific DNA aptamers, bypassing lengthy selection steps. The new approach significantly reduces the time and effort required for aptamer discovery.
Area of Science:
- Biotechnology
- Molecular Biology
- Microbiology
Background:
- Systematic Evolution of Ligands by Exponential Enrichment (SELEX) is a common method for aptamer isolation.
- Traditional SELEX involves multiple rounds of binding, separation, and amplification, which is time-consuming.
Purpose of the Study:
- To develop a streamlined method for isolating bacteria-specific DNA aptamers.
- To reduce the time and complexity associated with traditional SELEX.
Main Methods:
- A novel non-SELEX-based method was employed for aptamer isolation.
- The method involves serial removal of unbound DNA through centrifugation and a single round of separation and amplification.
Main Results:
- Bacteria-specific DNA aptamers were successfully isolated using the modified method.
- The process omitted the repeated binding, separation, and amplification steps characteristic of SELEX.
Conclusions:
- This non-SELEX approach offers a more efficient alternative for aptamer isolation.
- The streamlined method accelerates the discovery of bacteria-specific aptamers.
