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Related Experiment Video

Updated: Jan 1, 2026

Using Nanoplasmon-Enhanced Scattering and Low-Magnification Microscope Imaging to Quantify Tumor-Derived Exosomes
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Exosome aggregation mediated stop-flow paper-based portable device for rapid exosome quantification.

Boonta Chutvirasakul1,2,3, Nantana Nuchtavorn1, Leena Suntornsuk1

  • 1Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok, Thailand.

Electrophoresis
|December 18, 2019
PubMed
Summary

A new paper device rapidly quantifies exosomes using antibody-coated vesicles. This low-cost, portable method enables accurate exosome measurement in cell cultures without complex equipment.

Keywords:
AntiCD81Distance-based measurementExosome aggregationMicrofluidic paper-based devicePolydiacetylene

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Analytical Chemistry

Background:

  • Exosome quantification is crucial for understanding physiological and pathological processes.
  • Existing methods for exosome measurement can be complex, expensive, and require specialized expertise.

Purpose of the Study:

  • To develop a simple, rapid, and portable paper-based device for exosome quantification.
  • To utilize exosome-capture vesicles for a distance-based detection method.

Main Methods:

  • Conjugated polydiacetylene (PDA) with antiCD81 antibodies to create exosome-capture vesicles.
  • Optimized vesicle properties (size, ratio, concentration) and paper device design (reservoir diameter, lamination).
  • Developed a solvent migration assay where migration distance correlates with exosome concentration.

Main Results:

  • The device achieved linear correlation (R² > 0.98) for exosome concentrations from 10^6 to 10^10 particles/mL.
  • Exosome quantification in cell culture samples (COLO1, MDA-MB-231, HuR-KO1) was successfully demonstrated.
  • Results showed no statistically significant difference compared to nanoparticle tracking analysis (p > 0.05).

Conclusions:

  • A novel, low-cost, and rapid paper device for exosome quantification was successfully developed.
  • The portable platform requires minimal equipment and expertise, making exosome analysis more accessible.
  • This technology holds potential for quantifying other biomarker-related extracellular vesicles.