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Depth-sensitive Raman spectroscopy for skin wound evaluation in rodents
Joshua Weiming Su1,2,3, Qiang Wang1,4, Yao Tian1
1School of Chemical and Biomedical Engineering, Nanyang Technological University, 62 Nanyang Drive, 637459, Singapore.
Biomedical Optics Express
|December 20, 2019
Summary
This study introduces a new depth-sensitive Raman spectroscopy instrument for assessing rat skin wounds. The technology accurately differentiates between wound and healthy skin, improving with depth data for better wound diagnosis.
Area of Science:
- Biomedical Optics
- Spectroscopy
- Dermatology
Background:
- Raman spectroscopy shows promise for skin wound assessment.
- Skin's layered structure means biochemical changes in wound healing are depth-dependent.
- Depth-sensitive measurements can enhance Raman spectroscopy's utility for wound evaluation.
Purpose of the Study:
- To design, optimize, and evaluate a novel snapshot depth-sensitive Raman instrument.
- To enable depth-sensitive Raman measurements specifically for rat skin.
- To validate the system for preclinical wound assessment studies.
Main Methods:
- Developed a snapshot depth-sensitive Raman instrument with detailed optical design and optimization.
- Characterized performance using ex vivo porcine skin with varying layer thickness.
- Evaluated the system on ex vivo rat skin samples with induced wounds.
Main Results:
- Demonstrated successful depth-sensitive Raman measurements in rat skin.
- Statistical analysis confirmed the ability to differentiate between wound edges and healthy skin.
- Classification accuracy improved with the inclusion of data from multiple depths, indicating added value per depth.
Conclusions:
- The developed instrument enables snapshot depth-sensitive Raman measurements of rat skin.
- This technology supports enhanced skin wound assessment by utilizing depth-dependent biochemical information.
- The findings pave the way for in vivo preclinical studies of rat skin wounds.
