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Modulating Macrophage Phenotype by Sustained MicroRNA Delivery Improves Host-Implant Integration
Junquan Lin1, Ibrahim Mohamed2, Po Hen Lin1
1School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637459, Singapore.
Advanced Healthcare Materials
|December 20, 2019
Summary
Biomedical implant integration is improved by using microRNAs (miRs) to guide macrophage polarization. This strategy reduces fibrous capsule formation, enhancing implant function and host acceptance.
Area of Science:
- Biomaterials Science
- Immunology
- Regenerative Medicine
Background:
- Biomedical implant failure is often caused by the host's foreign body reaction (FBR).
- Fibrous capsule formation around implants impedes their normal function.
- Macrophage polarization significantly influences the FBR and host-implant integration.
Purpose of the Study:
- To investigate the use of microRNAs (miRs) delivered via nanofiber scaffolds to modulate macrophage polarization.
- To assess the impact of miR-induced macrophage polarization on fibrous capsule formation and host-implant integration.
Main Methods:
- Electrospun poly(caprolactone-co-ethyl ethylene phosphate) nanofiber scaffolds were fabricated.
- Scaffolds were loaded with M2-inducing miRs (Let-7c, miR-124) or an M1-inducing miR (Anti-Let-7c).
- These scaffolds were implanted into C57BL/6 mice to evaluate FBR and host-implant integration.
Main Results:
- M2-inducing miRs (Let-7c, miR-124) resulted in thinner fibrous capsules compared to Anti-Let-7c.
- miR-124 treatment group showed the highest blood vessel density within scaffolds.
- miR-encapsulated scaffolds enabled localized and sustained miR delivery, modulating macrophage polarization.
Conclusions:
- MicroRNA-loaded nanofiber scaffolds effectively modulate macrophage polarization.
- Targeting macrophage polarization with miRs can reduce fibrous capsule formation and improve host-implant integration.
- This approach offers a promising strategy for enhancing the long-term performance of biomedical implants.
