Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

EDTA: Auxiliary Complexing Reagents01:26

EDTA: Auxiliary Complexing Reagents

1.2K
EDTA titrations are usually carried out in highly basic conditions, where the fully deprotonated form of EDTA, Y4−, actively complexes with the free metal ions in the solution. Several metal ions precipitate as hydrous oxide (hydroxides, oxides, or oxyhydroxides) under these conditions, lowering the concentration of free metal ions in the solution. For this reason, auxiliary complexing agents or ligands such as ammonia, tartrate, citrate, or triethanolamine are used in EDTA titrations to...
1.2K
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

578
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
578
EDTA: Conditional Formation Constant01:09

EDTA: Conditional Formation Constant

1.9K
Each EDTA molecule has six binding sites: four carboxyl groups and two amino groups. The fully protonated form of EDTA is represented as H6Y2+. However, it can exist in different forms, H5Y+, H4Y, H3Y−, H2Y2−, and HY3−, depending on the pH of the solution. In very basic solutions with pH > 10.17, the fully deprotonated form, Y4−, is the predominant species that readily complexes with metal ions in a 1:1 ratio.
For the equilibrium reaction of the metal with the...
1.9K
Complexometric EDTA Titration Curves01:20

Complexometric EDTA Titration Curves

2.0K
EDTA titration curves determine the free metal ion concentration. The titration curve represents the change in concentration of free metal ions (p function) as a function of the volume of EDTA added. This curve consists of three regions: before, at, and after equivalence points. Excess free metal ions are present before the equivalence point. Equal concentrations of metal ions and EDTA are present at the equivalence point. After the equivalence point, excess EDTA exists. This means slight...
2.0K
EDTA: Direct, Back-, and Displacement Titration01:30

EDTA: Direct, Back-, and Displacement Titration

5.1K
The EDTA titration types for metal ion analysis include direct titration, back-titration, and replacement titration.
Direct titration involves buffering the metal ion solution to the desired pH and directly titrating with standard EDTA until the endpoint. The optimum pH ensures a large conditional formation constant of metal−EDTA and visibility of the free indicator color in the solution. In addition, auxiliary complexing reagents are used to prevent the precipitation of metal hydroxides...
5.1K
EDTA: Indirect and Alkalimetric Titration01:23

EDTA: Indirect and Alkalimetric Titration

1.7K
Unlike direct titration, back-titration, and displacement titration, indirect titration is an EDTA titration method for quantifying anions. In the indirect titration method, anions are precipitated as their insoluble salts with excess metal ions. The filtrate containing the excess metal ions is directly titrated with standard EDTA until the endpoint is achieved. Another approach involves extracting the metal ion and back-titrating with standard EDTA to obtain the endpoint. In this way, the...
1.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical, biological and cytometric characteristics of two patients with a homozygous A91V<i>PRF1</i> mutation.

Clinical & translational immunology·2026
Same author

Quantitative molecular breast imaging for early prediction of neoadjuvant systemic therapy response in locally advanced breast cancer patients.

Clinical imaging·2026
Same author

Inflammation-Driven Lymphoid Structures: Organization, Function, and Clinical Impact Across Autoimmunity, Cancer, and Checkpoint Toxicity.

Immunological reviews·2026
Same author

Clinical and pharmacological outcomes after switching from intravenous to subcutaneous infliximab in chronic inflammatory rheumatic diseases.

Joint bone spine·2026
Same author

Effect of structural connectivity on the assemblage diversity patterns of central Mexico icthyofauna.

Journal of fish biology·2026
Same author

[Sustainable healthcare transition: A guide for deprescribing unnecessary biological tests].

La Revue de medecine interne·2026
Same journal

[The role of the heart-on-a-chip to drug evaluation].

Annales de biologie clinique·2026
Same journal

A morphology-driven proof-of-concept study linking interphase nuclear abnormalities to dicentric-mediated genomic instability.

Annales de biologie clinique·2026
Same journal

Analytical validation and clinical concordance of Diazyme free light chain assays on the Cobas® platform compared with Freelite™.

Annales de biologie clinique·2026
Same journal

Small-cell secondary plasma cell leukemia mimicking low-grade B-cell lymphoma.

Annales de biologie clinique·2026
Same journal

[Multiple myeloma and analytical discrepancy in serum free light chain assay: a case report].

Annales de biologie clinique·2026
Same journal

[Summary of the 34th National Conference of the National College of Hospital Biochemistry].

Annales de biologie clinique·2026
See all related articles

Related Experiment Video

Updated: Jan 1, 2026

Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow
09:41

Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow

Published on: March 5, 2020

10.0K

[Stability study and external quality assessment scheme implementation for complement components dosage on EDTA

Benjamin Lopez1, Stéphanie Rogeau1, Anne-Sophie Deleplancque2

  • 1CHU Lille, Institut d'immunologie, Lille, France, Université de Lille, U995-LIRIC-Lille Inflammation Research International Center, Lille, France.

Annales De Biologie Clinique
|December 21, 2019
PubMed
Summary
This summary is machine-generated.

A new external quality assessment (EQA) scheme using EDTA plasma samples was developed in France. This inter-laboratory exchange (ILE) provides reliable quality control for complement testing, enhancing laboratory accreditation.

Keywords:
EDTA plasmacomplement componentsexternal quality assessmentquality control

More Related Videos

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
12:40

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

29.4K
Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
05:19

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

1.3K

Related Experiment Videos

Last Updated: Jan 1, 2026

Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow
09:41

Hemocompatibility Testing of Blood-Contacting Implants in a Flow Loop Model Mimicking Human Blood Flow

Published on: March 5, 2020

10.0K
Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System
12:40

Preparation and Pathogen Inactivation of Double Dose Buffy Coat Platelet Products using the INTERCEPT Blood System

Published on: December 7, 2012

29.4K
Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System
05:19

Author Spotlight: Advancing Protein Glycosylation Research Using a Fully Automated System

Published on: June 28, 2024

1.3K

Area of Science:

  • Clinical Chemistry
  • Immunology
  • Laboratory Medicine

Background:

  • External quality assessment (EQA) schemes are crucial for reliable laboratory diagnostics.
  • Currently, no EQA scheme in France utilizes EDTA plasma, the standard for complement analyte stability.
  • Laboratories often use non-standard matrices for EQA, potentially impacting result interpretation.

Purpose of the Study:

  • To establish a novel inter-laboratory exchange (ILE) program using EDTA plasma for complement EQA in France.
  • To provide a reliable quality control matrix aligned with routine clinical laboratory practice.
  • To support the accreditation of French laboratories performing complement analysis.

Main Methods:

  • Development of an ILE using pooled EDTA plasma from healthy donors.
  • Dilution of plasma to create distinct control levels for various complement analytes.
  • Validation through stability studies (storage conditions, sample matrix) and linearity assessments.
  • Focus on CH50, C3, C4, and C1-inhibitor measurements.

Main Results:

  • Demonstrated a four-week stability for prepared and stored ILE samples under specified conditions.
  • Confirmed the linearity of dilution steps for creating control levels.
  • Protocol validation confirmed suitability for ILE implementation constraints.
  • Successful implementation of the ILE program in January 2018.

Conclusions:

  • The developed ILE scheme using EDTA plasma is stable and reliable for complement analysis.
  • This program addresses the lack of suitable EQA matrices in France for EDTA plasma.
  • The ILE facilitates accurate quality assessment and supports laboratory accreditation for complement testing.