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Related Experiment Video

Updated: Jan 1, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

Published on: August 6, 2013

Enabling single cell electrical stimulation and response recording via a microfluidic platform.

Liwei Ni¹, Pawan Kc², Ge Zhang²

¹Department of Mechanical Engineering, University of Akron, Akron, Ohio 44325, USA.

Biomicrofluidics

|December 24, 2019

Summary

This study introduces a microfluidic device for precise electrical stimulation (ES) of single cells and recording their electrical field potential (EFP). The device enables continuous ES application and real-time EFP detection, revealing cell responses and neurotransmitter release.

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Last Updated: Jan 1, 2026

A Versatile Automated Platform for Micro-scale Cell Stimulation Experiments

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Published on: August 6, 2013

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Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

Designing Microfluidic Devices for Studying Cellular Responses Under Single or Coexisting Chemical/Electrical/Shear Stress Stimuli

Published on: August 13, 2016

Area of Science:

Biomedical Engineering
Cellular Electrophysiology
Neuroscience

Background:

Electrical stimulation (ES) influences cell behavior.
Studying single-cell electrophysiology requires advanced techniques.
Existing methods for ES and electrophysiological recording are often time-consuming.

Purpose of the Study:

To develop a microfluidic device for single-cell electrical stimulation and extracellular field potential (EFP) recording.
To enable continuous ES application and simultaneous EFP detection on individual cells.
To assess the device's efficacy in studying cellular responses to ES.

Main Methods:

A microfluidic device was designed with a constriction channel to trap single cells on an electrode.
Electrical stimulation (ES) was applied to trapped cells.
Extracellular field potential (EFP) was recorded from single human cardiomyocytes and primary rat cortex neurons during ES.
Glutamic acid secretion from stimulated neurons was measured.

Main Results:

The device successfully applied ES to single cells and recorded their EFP.
Excitable cells exhibited electrical responses upon ES.
Stimulated neurons showed increased glutamic acid secretion.
Cell viability remained unchanged after ES.

Conclusions:

The developed microfluidic device allows for continuous ES and accurate, rapid assessment of single-cell responses.
This technology offers a higher throughput method compared to traditional ES techniques.
The device is suitable for investigating the biological effects of ES on diverse cell types.