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High sensitivity detection of biotinylated molecules using a high-resolution resistive pulse sensor.

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This study introduces a new method for detecting biotinylated molecules using gold nanoparticle counting. The technique offers highly sensitive quantification, crucial for accurate diagnostics and biotechnology applications.

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Area of Science:

  • Nanotechnology
  • Biotechnology
  • Analytical Chemistry

Background:

  • Biotinylation is essential for molecular tagging in various biological applications.
  • Accurate detection of biotinylated molecules is critical for reliable assays and diagnostics.
  • Current detection methods may lack sensitivity or require extensive sample preparation.

Purpose of the Study:

  • To develop a novel, highly sensitive method for analyzing biotinylated targets.
  • To integrate microfluidic resistive pulse sensing with a competitive nanodimer assay.
  • To enable universal and precise quantification of biotinylated molecules.

Main Methods:

  • Utilized gold nanoparticle (GNP) counting with a microfluidic resistive pulse sensor.
  • Employed a competitive nanodimer formation assay involving biotin- and streptavidin-modified GNPs.
  • Quantified biotinylated targets by measuring the inhibition of nanodimer formation.

Main Results:

  • Demonstrated ultra-sensitive detection of biotinylated bovine serum albumin (BSA) down to 0.7606 pg mL⁻¹.
  • Showcased significant shifts in dimer ratio corresponding to minute changes in target concentration.
  • Validated the single-particle detection capability of the resistive pulse sensor.

Conclusions:

  • The novel strategy provides ultra-sensitive and accurate quantification of biotinylated molecules.
  • Minimal calibration and sample preparation are required, enhancing practicality.
  • This approach has broad potential in biotechnology, diagnostics, and tissue engineering.