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Area of Science:

  • Cell Biology
  • Biophysics
  • Nanotechnology

Background:

  • Cell surface charge is a critical regulator of cellular bio-functions.
  • Understanding single-cell surface charge distribution is essential for deciphering cell behaviors.
  • Existing methods may lack the resolution for single-cell surface charge mapping.

Purpose of the Study:

  • To develop and validate a method for mapping single-cell surface charge density.
  • To investigate the electrostatic interactions between cells and nanoparticles for charge mapping.
  • To quantify the surface charge distribution of different cell types.

Main Methods:

  • Utilized fluorescent nanoparticles (NPs) as probes for electrostatic cell-nanoparticle interactions.
  • Imaged and analyzed the fluorescence distribution of NPs on cell surfaces at varying vertical distances.
  • Established a correlation between fluorescence intensity and NP count to quantify surface charge density.
  • Tested the method on human umbilical vein endothelial cells (HUVECs) and HeLa cells.

Main Results:

  • Successfully mapped the surface charge distribution of individual HUVECs and HeLa cells.
  • Quantified the average zeta potentials for both cell types, showing good agreement with electrophoretic light scattering measurements.
  • Demonstrated the method's capability for rapid surface charge mapping of single cells.

Conclusions:

  • The developed electrostatic cell-nanoparticle interaction method provides a viable approach for single-cell surface charge mapping.
  • This technique offers advancements in cell-surface-charge characterization for various biomedical applications.
  • The method's accuracy and efficiency support its use in biological and nanomedical research.