Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Asynchronous DNA replication within the human beta-globin gene locus.

E Epner¹, W C Forrester, M Groudine

¹Fred Hutchinson Cancer Research Center, Seattle, WA 98104.

Proceedings of the National Academy of Sciences of the United States of America

|November 1, 1988

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

A phase II multicenter trial of hyperCVAD MTX/Ara-C and rituximab in patients with previously untreated mantle cell lymphoma; SWOG 0213.

Annals of oncology : official journal of the European Society for Medical Oncology·2013

Same author

G-CSF mobilized vs conventional donor lymphocytes for therapy of relapse or incomplete engraftment after allogeneic hematopoietic transplantation.

Bone marrow transplantation·2012

Same author

Bortezomib in patients with relapsed or refractory mantle cell lymphoma: updated time-to-event analyses of the multicenter phase 2 PINNACLE study.

Annals of oncology : official journal of the European Society for Medical Oncology·2008

Same author

Hematopoietic cell transplantation from related and unrelated donors after minimal conditioning as a curative treatment modality for severe paroxysmal nocturnal hemoglobinuria.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation·2003

Same author

The Ror receptor tyrosine kinase family.

Cellular and molecular life sciences : CMLS·2002

Same author

Targeting silence: the use of site-specific recombination to introduce in vitro methylated DNA into the genome.

Science's STKE : signal transduction knowledge environment·2001

Same journal

Chemotactic self-organization captures the dynamics of mammalian hair follicle patterning.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Tomographic imaging of superconducting order using particle-hole interference.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Inhibitory potential of autologous neutralizing antibodies sets quantitative limits on the rebound-competent HIV-1 reservoir.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Inferring epidemiological parameters under an infectious phylogeography model with visitor dynamics.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Analytical modeling for suction cup designs for skin-interfaced wearable devices.

Proceedings of the National Academy of Sciences of the United States of America·2026

Same journal

Improving cell-free metabolism through direct integration of artificial respiratory chains.

Proceedings of the National Academy of Sciences of the United States of America·2026

See all related articles

DNA replication timing of the human beta-globin gene locus was analyzed. Specific regions within the locus replicate later in S phase, potentially indicating replication termination sites.

Area of Science:

Genetics
Molecular Biology
Cell Biology

Background:

The human beta-globin gene locus is crucial for red blood cell development.
Understanding DNA replication timing is vital for gene regulation and genome stability.

Purpose of the Study:

To investigate the DNA replication timing of the human beta-globin gene locus.
To identify specific regions with distinct replication patterns within the locus.

Main Methods:

Utilized blot hybridization with bromodeoxyuridine (BrdUrd)-substituted DNA.
Analyzed DNA from different S phase stages in K562 and Manca cell lines.
Employed probes spanning over 120 kilobases of the beta-globin gene locus.

Main Results:

Related Experiment Videos

The majority of the beta-globin gene locus replicates in early S phase in K562 cells.
Replication occurs in middle-to-late S phase in Manca cells.
Three small regions within the locus and one in the alpha-globin region of K562 cells replicate later in S phase.

Conclusions:

Identified distinct replication timing patterns for specific regions within the human beta-globin gene locus.
These late-replicating regions may function as replication termination sites.
Replication timing differences highlight locus complexity and potential regulatory mechanisms.