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Updated: Jan 1, 2026

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
Published on: January 7, 2020
Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular proteins
Jase Gehring1,2, Jong Hwee Park2, Sisi Chen2
1Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.
Abstract:
We describe a universal sample multiplexing method for single-cell RNA sequencing in which fixed cells are chemically labeled by attaching identifying DNA oligonucleotides to cellular proteins. Analysis of a 96-plex perturbation experiment revealed changes in cell population structure and transcriptional states that cannot be discerned from bulk measurements, establishing an efficient method for surveying cell populations from large experiments or clinical samples with the depth and resolution of single-cell RNA sequencing.
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