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Characterization of a new murine monoclonal antibody against human DP antigens
V Horejsí1, B Chorváth, K Polákova
1Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Tissue Antigens
|July 1, 1988
Abstract:
Murine Hybridoma cell line BraFB6 was prepared producing monoclonal antibody reactive with an antigen identified as DP. This identification is based on the results of immunoprecipitation from radiolabeled cell lysates, competitive binding-inhibition assay with a reference anti-DP antibody B7/21, sequential immunoprecipitation and specific binding to the purified DP glycoprotein. In addition to this anti-DP monoclonal antibody, two anti-DR and two anti-DR +DP monoclonals were obtained.