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Multi targeted cell membrane chromatography: A comprehensive method for screening the anaphylactoid components from

Yuanyuan Lin¹, Qianqian Jia², Wei Sun²

¹School of Pharmacy, Xi'an Jiaotong University, 76# Yanta West Road, Xi'an, 710061, China; National-provincial Joint Engineering Research Center for National Vascular Medicine Screening & Analysis, Xi'an, 710061, China; School of Pharmacy and Department of Chemistry, University of Wisconsin-Madison, Madison, WI, 53705, USA.

|January 2, 2020

Summary

This summary is machine-generated.

Screening anaphylactoid drug components is challenging. A novel multi-target cell membrane chromatography coupled with LC-MS effectively identifies anaphylactoid ingredients like ginsenosides in complex samples, aiding drug safety.

Keywords:

Anaphylactoid components Complex samples Multi targeted cell membrane chromatography Shenmai injection

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Area of Science:

Pharmacology
Analytical Chemistry
Biochemistry

Background:

Anaphylactoid reactions from drugs pose significant health risks.
Identifying causative agents in complex pharmaceutical formulations is difficult.
Existing screening methods lack comprehensiveness for anaphylactoid components.

Purpose of the Study:

To develop and validate a comprehensive method for screening anaphylactoid ingredients in complex samples.
To identify specific anaphylactoid components within Shenmai Injection.
To assess the anaphylactoid potential of identified components.

Main Methods:

Development of a multi-targeted cell membrane chromatography (MCM) system.
Online coupling of MCM with Liquid Chromatography-Mass Spectrometry (LC-MS).
Utilizing Mas-related G protein coupled receptor X2, Fc epsilon RI, and H1 receptors for target enrichment.
In vitro sensitization assays for verifying anaphylactoid effects.

Main Results:

The developed MCM-LC-MS system demonstrated high selectivity and suitability for screening.
Five anaphylactoid components were identified in Shenmai Injection: Ginsenoside Rb1, Rb2, Rc, Rd, and 20(S)-ginsenoside Rg3.
In vitro assays confirmed the anaphylactoid effects of these identified ginsenosides.

Conclusions:

The MCM-LC-MS system offers a robust solution for comprehensive screening of anaphylactoid components in complex matrices.
This method provides valuable insights for enhancing drug quality control and ensuring patient safety.
The identified ginsenosides warrant further investigation regarding their role in drug-induced anaphylactoid reactions.