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Updated: Dec 31, 2025

Optimization and Comparative Analysis of Plant Organellar DNA Enrichment Methods Suitable for Next-generation Sequencing
Published on: July 28, 2017
Methods and Tools for Plant Organelle Genome Sequencing, Assembly, and Downstream Analysis
Sanand Sandhya1, Harsha Srivastava1, Tanvi Kaila1
1ICAR-National Institute for Plant Biotechnology, New Delhi, India.
Abstract:
Organelles play an important role in a eukaryotic cell. Among them, the two organelles, chloroplast and mitochondria, are responsible for the critical function of photosynthesis and aerobic respiration. Organellar genomes are also very important for plant systematic studies. Here we have described the methods for isolation of the mitochondrial and plastid DNA and its subsequent sequencing with the help of NGS technology. We have also discussed in detail the various tools available for assembly, annotation, and visualization of the organelle genome sequence.
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