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Cytologic Evaluation of Tumor-Infiltrating Lymphocytes for Adoptive Cell Therapy
Sara E Monaco1, Liron Pantanowitz1, Juan Xing1
1Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA.
Objectives:
Novel immunotherapeutic options for refractory metastatic cancer patients include adoptive cell therapies such as tumor infiltrating lymphocytes (TILs). This study characterizes the clinicopathologic findings in a cohort of TIL specimens.
Methods:
Patients with metastatic malignancy who were eligible had TILs from their metastases grown and expanded and then sent to pathology.
Results:
A total of 11 TIL specimens (10 melanoma, 1 adenocarcinoma) from patients enrolled in an experimental clinical trial were reviewed. All specimens showed more than 200 lymphoid cells, stained positive for lymphoid markers confirming an activated cytotoxic T-cell immunophenotype, and morphologically showed an intermediate-sized population with immature chromatin and frequent mitoses. Six cases (55%) showed large cells with nucleomegaly and prominent nucleoli.
Conclusions:
This report is the first describing cytopathologic findings of autologous TIL therapy including adequacy guidelines and expected cytomorphologic and immunophenotypic findings. To meet this novel clinical demand, a predefined cytology protocol to rapidly process and interpret these specimens needs to be established.
