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LncRNA DLGAP1-AS2 modulates glioma development by up-regulating YAP1 expression

Wei Miao¹, Ning Li¹, Bin Gu¹

¹Department of Neurosurgery, Zhongda Hospital, Southeast University, 87 DingJiaQiao Road, Gulou District, Nanjing, Jiangsu 210009, China.

Journal of Biochemistry

|January 4, 2020

Summary

Long non-coding RNA DLGAP1-AS2 is upregulated in glioma, inhibiting tumor growth and progression. This study reveals DLGAP1-AS2 targets YAP1, offering potential therapeutic strategies for glioma treatment.

Area of Science:

Oncology
Molecular Biology
Genetics

Background:

Long non-coding RNAs (lncRNAs) play crucial roles in various biological processes, but the function of DLGAP1-AS2 in glioma remains largely unknown.
Glioma is a common primary brain tumor with a poor prognosis, necessitating the identification of novel therapeutic targets.

Purpose of the Study:

To investigate the role of lncRNA DLGAP1-AS2 in glioma progression.
To elucidate the underlying molecular mechanisms of DLGAP1-AS2 in glioma.
To explore DLGAP1-AS2 as a potential therapeutic target for glioma.

Main Methods:

Quantitative real-time PCR to assess DLGAP1-AS2 expression in glioma tissues and cell lines.
Cell proliferation, migration, and apoptosis assays (e.g., EdU, Transwell, flow cytometry) to evaluate the functional impact of DLGAP1-AS2.
Western blotting to detect YAP1 protein levels.
In vivo xenograft tumor models to assess the effect of DLGAP1-AS2 knockdown on tumor growth.

Main Results:

DLGAP1-AS2 was significantly upregulated in glioma tissues and correlated with poor patient prognosis.
Depletion of DLGAP1-AS2 inhibited glioma cell proliferation and migration while promoting apoptosis in vitro.
Knockdown of DLGAP1-AS2 suppressed xenograft glioma tumor growth in vivo.
DLGAP1-AS2 was found to regulate the expression of Yes Associated Protein 1 (YAP1), a key mediator of cell proliferation, migration, and apoptosis in glioma.

Conclusions:

lncRNA DLGAP1-AS2 plays a critical oncogenic role in glioma progression by regulating YAP1.
DLGAP1-AS2 represents a potential diagnostic biomarker and therapeutic target for glioma.

Keywords:

DLGAP1-AS2 LncRNA YAP1 glioma

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