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Metabolic adjustments during compatible interaction between barley genotypes and stripe rust pathogen
Prabhjot Singla1, Rachana D Bhardwaj1, Simarjit Kaur2
1Department of Biochemistry, Punjab Agricultural University, Ludhiana, 141004, India.
Plant Physiology and Biochemistry : PPB
|January 6, 2020
Summary
Barley plants resist stripe rust by increasing cell wall components like lignin and accumulating defense compounds such as proline. These biochemical changes, involving specific enzymes, are key indicators of barley
Area of Science:
- Plant Pathology
- Biochemistry
- Molecular Biology
Background:
- Stripe rust, caused by *Puccinia striiformis*, significantly impacts global barley production.
- Understanding the biochemical mechanisms of barley resistance is crucial for developing resistant cultivars.
Purpose of the Study:
- To investigate the role of specific enzymes and metabolites in barley's resistance to stripe rust.
- To identify potential biochemical markers for assessing plant defense status against this pathogen.
Main Methods:
- Comparative analysis of enzyme activities and metabolite levels in resistant (RD2901) and susceptible (Jyoti) barley genotypes after infection.
- Quantification of β-glucan, PR proteins, phenylalanine ammonia lyase (PAL), tyrosine ammonia lyase (TAL), diamine oxidase (DAO), polyamine oxidase (PAO), glutamate dehydrogenase (GDH), ornithine aminotransferase (OAT), proline, glycine betaine, choline, and lignin.
Main Results:
- Resistant barley (RD2901) showed elevated PR proteins, PAL, TAL, β-glucan, and lignin, alongside enhanced GDH and OAT activities and increased proline, glycine betaine, and choline.
- Sensitive barley (Jyoti) exhibited limited increases in PAL, OAT, lignin, and choline, indicating a weaker defense response.
- Lignin accumulation was significantly lower in the susceptible genotype compared to the resistant one.
Conclusions:
- Key enzymes and metabolites, including those in phenol and proline metabolism, play a vital role in barley's defense against stripe rust.
- These identified biochemical markers can be utilized for characterizing plant defensive states and aiding crop protection strategies.
