Electrochemical endotoxin aptasensor based on a metal-organic framework labeled analytical platform

Area of Science:

• * Biosensors and Nanomaterials
• * Electrochemical Sensing
• * Biomedical Diagnostics

Background:

• * Accurate detection of lipopolysaccharide (LPS) is crucial for diagnosing Gram-negative bacterial infections.
• * Existing detection methods often lack sensitivity, specificity, or require complex procedures.
• * Development of rapid, sensitive, and selective biosensors is highly desirable.

Purpose of the Study:

• * To construct a novel electrochemical aptasensor for sensitive and selective LPS detection.
• * To utilize copper-based metal-organic framework (Cu-MOF) as a label and DNA aptamers as recognition elements.
• * To investigate the sensor's performance, stability, and applicability in real samples.

Main Methods:

• * Synthesis of carboxyl-functionalized polypyrrole nanowires (PPy NWs) via electrochemical polymerization.
• * Immobilization of LPS-specific DNA aptamers onto PPy NWs.
• * Labeling with Cu-MOF and electrochemical detection using differential pulse voltammetry (DPV).

Main Results:

• * The aptasensor demonstrated high sensitivity for LPS detection within a range of 1.0 pg/mL to 1.0 ng/mL.
• * Achieved a low detection limit of 0.29 pg/mL for LPS.
• * Exhibited excellent selectivity, stability, and regeneration capabilities, successfully detecting LPS in tap water samples.

Conclusions:

• * The developed electrochemical aptasensor offers a promising platform for sensitive and selective LPS detection.
• * The combination of Cu-MOF and DNA aptamers provides an effective strategy for biosensor development.
• * The sensor's performance in real samples highlights its potential for practical diagnostic applications.