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Updated: Dec 31, 2025
A Tandem Liquid Chromatography–Mass Spectrometry-based Approach for Metabolite Analysis of Staphylococcus aureus
Published on: March 28, 2017
Rolling circle amplification based colorimetric determination of Staphylococcus aureus.
Yanan Li1, Junying Wang2, Shuo Wang3
1State Key Laboratory for Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, 29 The Thirteenth Road, Tianjin Economy and Technology Development Area, Tianjin, 300457, People's Republic of China.
This study presents a sensitive colorimetric assay for detecting Staphylococcus aureus DNA. The method utilizes rolling circle amplification and gold nanoparticles for enhanced detection, achieving low limits for target DNA and genomic DNA.
Area of Science:
- Molecular Biology
- Analytical Chemistry
- Biotechnology
Background:
- Accurate detection of Staphylococcus aureus is crucial for food safety and clinical diagnostics.
- Existing methods may lack the sensitivity or speed required for rapid screening.
Purpose of the Study:
- To develop a highly sensitive and specific colorimetric microplate assay for Staphylococcus aureus DNA detection.
- To validate the assay's performance using spiked food samples.
Main Methods:
- Design of linear padlock probes for specific target sequence recognition.
- Circularization by ligation, followed by rolling circle amplification (RCA) initiated by biotin-labeled capture probes.
- Signal amplification using gold nanoparticle (AuNP)-antibody conjugates and a colorimetric readout via HRP catalysis.
Main Results:
- Achieved a detection limit of 1.2 pM for target sequences and 7.4 pg/µL for genomic DNA.
- Demonstrated successful application by analyzing spiked food samples, confirming the method's potential.
- The assay leverages RCA and AuNP-based signal transduction for enhanced sensitivity.
Conclusions:
- The developed colorimetric assay offers a sensitive and reliable method for Staphylococcus aureus DNA detection.
- The assay's high sensitivity and applicability to food samples highlight its potential for diagnostic and screening purposes.
