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Immobilization of Cells on Polyurethane Foam
Ignacio de Ory1, Gema Cabrera1, Martin Ramirez1
1Department of Chemical Engineering and Food Technology, Faculty of Sciences, University of Cadiz, Cadiz, Spain.
Methods in Molecular Biology (Clifton, N.J.)
|January 16, 2020
Summary
This study details methods for immobilizing model cells onto polyurethane foam carriers for use in reactors. These protocols enhance cell containment and recovery in both batch and continuous systems.
Area of Science:
- Biotechnology
- Chemical Engineering
- Materials Science
Background:
- Polyurethane foam is a cost-effective carrier with favorable properties like high porosity and surface area.
- Cell immobilization is crucial for efficient use in laboratory and industrial reactors.
Purpose of the Study:
- To provide detailed protocols for immobilizing model cells onto polyurethane foam.
- To enable the use of immobilized cell systems in various reactor configurations.
Main Methods:
- Two distinct immobilization protocols are presented: one for stirred tank reactors (batch) and another for packed column reactors (continuous).
- Methods cover carrier sterilization, analytical techniques, and the immobilization process itself.
Main Results:
- The described protocols facilitate the immobilization of model cells onto polyurethane foam carriers.
- The methods are applicable to both discontinuous (batch) and continuous reactor operation modes.
Conclusions:
- Polyurethane foam offers a practical and economical solution for cell immobilization in bioreactors.
- The provided protocols support the implementation of immobilized cell technology in diverse industrial and laboratory settings.
