Effect of casein phosphopeptide-amorphous calcium phosphate as a remineralizing agent - An In Vivo study

Demineralization and remineralization have a crucial impact on the hardness and strength of teeth. Casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) Trident white chewing gum has anticariogenic property and also stimulates saliva, which buffers the oral cavity and promotes remineralization. Trident sugar-free gum, therefore, is an excellent delivery vehicle for promoting enamel remineralization.

The aim of this study is to show that CPP-ACP-containing chewing gum would increase the level of calcium concentration of saliva, thereby supplying calcium and phosphorus to whole dentition for a prolonged period and aid in remineralization of tooth surfaces.

An in vivo nonrandomized clinical trial study was carried among 60 children. Unstimulated saliva from each 60 selected participants was collected. Then each participant was given two pellets of chewing gum containing CPP-ACP and asked to chew for a period of 20 min, after which saliva samples were again collected from each participant. The study was carried out for 15 days, and at three intervals, calcium and phosphorus levels were assessed using affiliated reagent kits and spectrophotometer.

Significant difference was found in the calcium and phosphorus concentration of saliva before and after chewing CPP-ACP-containing chewing gum. When post calcium and phosphate levels were analyzed among different time intervals, a highly statistically significant difference was observed (P = 0.000).

Clinical trial study shows that chewing sugar-free gum containing CPP-ACP can be regarded as an additional caries prevention tool.