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Updated: Dec 30, 2025

Conducting Multiple Imaging Modes with One Fluorescence Microscope
Published on: October 28, 2018
Design of Microscope Optics for the Acquisition of Multiple Wavelength Band Image
Abstract:
We design combinations of filters that lead to make outputs of multiple wavelength bands simultaneously. These bands are either 1) Infrared, RGB, Ultraviolet-3ranges 2) IR, Red, Green, Blue, UV-5ranges 3) IR+ Red, Green, Blue+UV-3ranges. Each is mostly devised by the arrangement of normal cold mirror, UV cold mirror, Philips(RGB) prism, UV filter and color filter. For the selection 1), UV cold mirror reflects UV and transmit VIS + IR. VIS and IR are sorted by normal cold mirror, which passes IR and reflects VIS. Each sorted light goes to each sensor to capture the images. The sorting of VIS and UV also can be done by UV filter. For the selection 2), have a same process in 1) but make VIS pass Philips(RGB) prism which sorts VIS into R, G, B. For the selection 3), the light passes green color filter first, Green color is only transmitted and the others are reflected. And then reflected light goes through UV cold mirror so that UV and Blue (wavelength <; 500nm) are reflected. And the others(wavelength>550nm, but green color zone was already sorted roughly wavelength>600nm) are transmitted, which is Red and IR. This sorting can be done by another design. After sorting all ranges by the selection 2), we can arrange UV goes a same pass with blue, and IR with red. We need band pass filters (in here, color filters) to revise the passes.
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