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Updated: Dec 30, 2025

Specific Labeling of Mitochondrial Nucleoids for Time-lapse Structured Illumination Microscopy
Published on: June 4, 2020
A novel water-soluble quinoline-indole derivative as a three-photon fluorescent probe for identifying nucleolus RNA
Imad Elddin Haj Elhussin1, Sijing Zhang1, Jiejie Liu2
1Department of Chemistry, Key Laboratory of Functional Inorganic Material Chemistry of Anhui Province, Anhui University, Hefei 230601, P. R. China. zhangqiong.314@163.com yptian@ahu.edu.cn.
Abstract:
We present the design and synthesis of water-soluble quinoline-indole-based derivatives (IM-1, IM-2, and IM-3) with three-photon absorption activity. IM-3 can specifically target DNA and RNA accompanied by an obvious three-photon fluorescence enhancement in the second near-infrared window (1000-1700 nm). The in vitro experiments demonstrate that IM-3 can simultaneously stain mitochondria and the nucleolus both in living and fixed cells. The organelle-specific targeting behaviour is successfully visualized under stimulated emission depletion (STED) nanoscopy.

