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Related Experiment Video

Updated: Dec 30, 2025

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Efficient PAM-Less Base Editing for Zebrafish Modeling of Human Genetic Disease with zSpRY-ABE8e

Published on: February 17, 2023

Efficient base editing with high precision in rabbits using YFE-BE4max.

Zhiquan Liu¹, Siyu Chen¹, Huanhuan Shan¹

¹Key Laboratory of Zoonosis Research, Ministry of Education, College of Animal Science, Jilin University, Changchun, 130062, China.

Cell Death & Disease

|January 22, 2020

Summary

This summary is machine-generated.

A new base editor, YFE-BE4max, offers high efficiency and precision for C-to-T conversion in rabbits. This tool precisely models human diseases by enabling targeted gene mutations.

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Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Production of Apolipoprotein C-III Knockout Rabbits using Zinc Finger Nucleases

Published on: November 18, 2013

Area of Science:

Molecular Biology
Gene Editing
Biotechnology

Background:

Cytidine base editors facilitate C-to-T conversion but face efficiency-precision trade-offs.
Existing base editors require improvement for precise genome editing applications.

Purpose of the Study:

To develop a novel base editor with enhanced precision and maintained efficiency.
To create a tool for precise modeling of human genetic diseases in rabbits.

Main Methods:

Rational mutation of the cytidine deaminase domain.
Development and application of the YFE-BE4max base editor in rabbits.
Generation of specific mutations (Tyr p. Q68Stop, Lmna p. G607G) to model human diseases.

Main Results:

YFE-BE4max narrowed the editing width to approximately three nucleotides with high efficiency.
Successfully mediated Tyr p. Q68Stop and Lmna p. G607G mutations in F0 rabbits.
Precisely recapitulated human diseases Oculocutaneous Albinism type 1 (OCA1) and Hutchinson-Gilford Progeria Syndrome (HGPS).

Conclusions:

YFE-BE4max provides a precise and efficient base editing tool for rabbit models.
Enhances the capacity for accurate modeling of human genetic diseases.
Offers a promising system for advanced gene editing applications.