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Trimethylation and Differential Mobility Spectroscopy in Quantitative Peptide Analysis: Increasing Selectivity and
Voislav Blagojevic1, Diethard K Bohme1
1Department of Chemistry and Centre for Research in Mass Spectrometry, York University, 4700 Keele Street, Toronto, ON M3J 1P3 (Canada), Fax: (+1) 416-736-5936.
Abstract:
Isotopic labeling of peptides by trimethylation creates a charged quaternary amine group on the peptide that provides clear differentiation from unlabeled protonated peptides and protonated or sodiated chemical background. Differential mobility spectrometry, with its use of a chemical modifier, allows otherwise undesirable ion/molecule reactions in the mobility cell to increase selectivity and sensitivity of quantitative peptide analysis. A high proton affinity modifier selectively removes protonated and sodiated interference and background ions by proton and sodium transfer, while leaving the trimethylated ions with their quaternary amine groups unchanged.
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