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Related Experiment Video

Updated: Dec 29, 2025

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

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Published on: April 19, 2010

Flow-Free Microfluidic Device for Quantifying Chemotaxis in Spermatozoa.

Johanna T W Berendsen¹, Stella A Kruit¹, Nihan Atak¹

¹BIOS-Lab on a Chip Group, MESA+ Institute for Nanotechnology and MIRA Institute for Biomedical Technology and Technical Medicine , University of Twente , 7500 AE Enschede , The Netherlands.

Analytical Chemistry

|January 30, 2020

Summary

This study introduces a novel microfluidic chip for assessing sperm chemotaxis, a key factor in fertilization. Boar sperm demonstrated attraction to progesterone gradients, suggesting a new diagnostic approach for male fertility.

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Last Updated: Dec 29, 2025

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

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Published on: April 19, 2010

Two Types of Assays for Detecting Frog Sperm Chemoattraction

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Published on: December 27, 2011

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Microfluidic Platform for Measuring Neutrophil Chemotaxis from Unprocessed Whole Blood

Published on: June 3, 2014

Area of Science:

Reproductive Biology
Biomedical Engineering
Microfluidics

Background:

Current male fertility diagnostics primarily assess semen parameters like sperm count, volume, and motility.
Sperm's chemotactic ability, its response to chemical gradients, is an underutilized factor influencing fertilization success.

Purpose of the Study:

To develop and validate a simple, flow-free microfluidic chip for evaluating sperm chemotaxis.
To investigate the chemotactic response of boar spermatozoa to progesterone.

Main Methods:

Fabrication of a microfluidic chip using a hybrid hydrogel (8% gelatin/1% agarose).
Conducting chemotaxis experiments by exposing boar spermatozoa to a 1 μM progesterone gradient within the microfluidic chip.

Main Results:

The microfluidic chip was successfully fabricated and demonstrated ease of use.
Boar spermatozoa exhibited a significant attraction response towards the progesterone gradient, confirming chemotaxis.

Conclusions:

The developed microfluidic chip offers a straightforward method for assessing sperm chemotactic behavior.
Progesterone acts as a chemoattractant for boar spermatozoa, highlighting its potential role in fertility and diagnostics.