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Updated: Dec 29, 2025

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
Published on: November 3, 2020
CRISPR/Cas9-Mediated Genome Engineering of Primary Human B Cells
Kanut Laoharawee1, Matthew J Johnson1, Branden S Moriarity2
1Department of Pediatrics, Cardiovascular Research, Masonic Cancer Center, Center for Genome Engineering, University of Minnesota, Minneapolis, MN, USA.
Abstract:
The CRISPR/Cas9 system allows for site-specific gene editing and genome engineering of primary human cells. Here we describe methods for gene editing and genome engineering of B cells isolated from human peripheral blood mononuclear cells using CRISPR/Cas9. Editing frequencies of up to 90% and integration rates greater than 60% can be achieved with this method.
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