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Serum amphotericin-B assay by scanning spectrophotometer
Z K Shihabi1, B L Wasilauskas, J E Peacock
1Department of Pathology, Wake Forest University, Bowman Gray School of Medicine, Winston-Salem, North Carolina.
Therapeutic Drug Monitoring
|January 1, 1988
Summary
A new 3-minute assay accurately measures amphotericin-B levels in serum. This simple method uses spectral scanning and correlates well with established high-performance liquid chromatography techniques.
Area of Science:
- Analytical Chemistry
- Clinical Chemistry
- Pharmacology
Background:
- Amphotericin-B is a critical antifungal medication.
- Accurate therapeutic drug monitoring is essential for optimizing amphotericin-B treatment and minimizing toxicity.
- Existing methods for amphotericin-B quantification can be time-consuming or require specialized equipment.
Purpose of the Study:
- To develop and validate a rapid and specific assay for quantifying amphotericin-B in human serum.
- To establish a cost-effective and accessible method for therapeutic drug monitoring of amphotericin-B.
Main Methods:
- Serum samples were extracted with acetonitrile.
- Amphotericin-B concentration was determined by measuring absorbance at 408 nm using spectral scanning between 450 and 350 nm.
- The developed assay was compared against a high-performance liquid chromatography (HPLC) method.
Main Results:
- The assay demonstrated specificity for amphotericin-B.
- The assay required only 3 minutes for completion.
- Results from the new assay showed strong correlation with the established HPLC method, indicating good accuracy and reliability.
Conclusions:
- A simple, rapid, and specific spectrophotometric assay for amphotericin-B in serum has been developed.
- This assay provides a reliable alternative for therapeutic drug monitoring of amphotericin-B.
- The method's speed and simplicity make it suitable for routine clinical laboratory use.