Related Experiment Video
Updated: Dec 29, 2025
11:44
Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
Published on: March 30, 2019
7.9K
Long non‑coding RNA GAS5 increases the radiosensitivity of A549 cells through interaction with the miR‑21/PTEN/Akt
Li Chen1, Ping Ren2, Yandong Zhang3
1Jilin Provincial Key Laboratory of Tooth Development and Bone Remodeling, Department of Oral Radiology, School and Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.
Oncology Reports
|February 6, 2020
Summary
Long non-coding RNA GAS5 enhances radiosensitivity in non-small cell lung cancer (NSCLC) by downregulating miR-21, increasing PTEN, and suppressing Akt phosphorylation. This suggests GAS5 as a potential therapeutic target for improving NSCLC radiotherapy outcomes.
Area of Science:
- Molecular Biology
- Oncology
- Genetics
Background:
- Radiotherapy resistance is a major challenge in non-small cell lung cancer (NSCLC) treatment.
- Long non-coding RNAs (lncRNAs) are implicated in various cellular processes, but their role in NSCLC radiosensitivity remains unclear.
Purpose of the Study:
- To investigate the role of lncRNAs in NSCLC radiosensitivity.
- To elucidate the molecular mechanisms by which lncRNAs modulate radiosensitivity in NSCLC.
Main Methods:
- RNA sequencing (RNA-Seq) to identify differentially expressed lncRNAs.
- Quantitative real-time PCR (qPCR) to validate lncRNA expression.
- Cell apoptosis assays and Western blotting to assess molecular changes.
Main Results:
- lncRNA GAS5 was significantly upregulated in radiosensitive NSCLC cells (NCI-H460) upon ionizing radiation (IR) exposure.
- Overexpression of GAS5 decreased miR-21 levels and enhanced IR-induced apoptosis in NSCLC cells.
- GAS5 increased PTEN expression and suppressed Akt phosphorylation via miR-21 modulation, indicating an interaction with the miR-21/PTEN/Akt axis.
Conclusions:
- lncRNA GAS5 exhibits a radiosensitizing effect in NSCLC.
- GAS5 holds potential as a therapeutic target to enhance the efficacy of radiotherapy in NSCLC treatment.
Related Concept Videos
02:39lncRNA - Long Non-coding RNAs
9.6K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.6K
02:39lncRNA - Long Non-coding RNAs
3.4K
3.4K
01:22MicroRNAs
3.6K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.6K
01:22MicroRNAs
23.8K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
23.8K
03:03mTOR Signaling and Cancer Progression
4.5K
The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
The mTOR pathway or the...
4.5K
02:23Abnormal Proliferation
5.0K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K