Related Experiment Video
Updated: Dec 29, 2025
10:00
Targeting Biofilm Associated Staphylococcus aureus Using Resazurin Based Drug-susceptibility Assay
Published on: May 5, 2016
13.7K
Biofilm formation by clinically isolated Staphylococcus Aureus from India
Alasthimannahalli Gangadhara Triveni1, Mendem Suresh Kumar2, Chavadi Manjunath3
1Gulbarga University, Kalaburagi-585106, Karnataka, India. triveni.ag@gmail.com.
Journal of Infection in Developing Countries
|February 7, 2020
Summary
Staphylococcus aureus clinical isolates frequently form biofilms, a key factor in infections. The Microtiter plate method is most effective for detecting biofilm production in these staphylococcal strains.
Area of Science:
- Microbiology
- Infectious Diseases
Background:
- Staphylococcal biofilms are a major cause of hospital and community-acquired infections worldwide.
- Understanding biofilm formation is crucial for combating staphylococcal pathogenesis.
Purpose of the Study:
- To investigate biofilm formation in Staphylococcus aureus clinical isolates.
- To compare different methods for assessing biofilm production.
Main Methods:
- Screened 188 Staphylococcus aureus clinical isolates for biofilm formation.
- Utilized Congo red Agar, Tube, and Microtiter plate methods for qualitative and quantitative assessment.
- Performed statistical analysis to determine method sensitivity and specificity.
Main Results:
- 72 (38.29%) of isolates were biofilm producers (34 strong, 38 moderate).
- Biofilm formation was highest in pus (39.06%), blood (38.23%), and urine (34.61%) isolates.
- The Microtiter plate method demonstrated superior sensitivity and specificity for screening biofilm production.
Conclusions:
- Biofilm formation is a significant virulence factor in staphylococcal infections.
- Microtiter plate and Congo red agar assays are reliable for biofilm estimation.
- Monitoring biofilm formation aids in assessing infection severity.
