Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

2.7K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
2.7K
X-ray Diffraction of Biological Samples01:10

X-ray Diffraction of Biological Samples

4.6K
X-ray diffraction or XRD is an analytical tool that utilizes X-rays to study ordered structures such as crystalline organic and inorganic samples, polycrystalline materials, proteins, carbohydrates, and drugs.
According to Bragg's law, when X-rays strike the sample positioned on a stage, the rays are  scattered by the electron clouds around the sample atoms. The  X-ray diffraction or scattering is caused by constructive interference of the X-ray waves that reflect off the internal...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author Correction: A dense ring of the trans-Neptunian object Quaoar outside its Roche limit.

Nature·2024
Same author

A dense ring of the trans-Neptunian object Quaoar outside its Roche limit.

Nature·2023
Same author

WhatEELS. A python-based interactive software solution for ELNES analysis combining clustering and NLLS.

Ultramicroscopy·2021
Same author

Support vector machine for EELS oxidation state determination.

Ultramicroscopy·2020
Same author

Quasi-parallel precession diffraction: Alignment method for scanning transmission electron microscopes.

Ultramicroscopy·2018
Same author

Is training sufficient for ultrasound operators to diagnose deep infiltrating endometriosis and bowel involvement by transvaginal ultrasound?

Journal of gynecology obstetrics and human reproduction·2018
Same journal

Predictive drift compensation of multi-frame STEM via live scan modification.

Ultramicroscopy·2026
Same journal

Deep PACBED: Multitask analysis of PACBED images using deep neural networks.

Ultramicroscopy·2026
Same journal

Guided progressive reconstructive imaging: A new quantization-based framework for low-dose, high-throughput and real-time analytical ptychography.

Ultramicroscopy·2026
Same journal

Brightness optimization in a 200 keV DTEM source by geometry-driven aberration suppression.

Ultramicroscopy·2026
Same journal

Characterization of the Timepix4 hybrid pixel detector and its impact on four-dimensional scanning transmission electron microscopy (4D-STEM).

Ultramicroscopy·2026
Same journal

Contamination analysis of the residual gas composition in transmission electron microscopy.

Ultramicroscopy·2026
See all related articles

Related Experiment Video

Updated: Dec 29, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

5.7K

Fast-ADT: A fast and automated electron diffraction tomography setup for structure determination and refinement.

S Plana-Ruiz1, Y Krysiak2, J Portillo3

  • 1Institut für Angewandte Geowissenschaften, Technische Universität Darmstadt, Petersenstrasse 23, 64287 Darmstadt, Germany; LENS, MIND/IN2UB, Departament d'Enginyeria Electrònica i Biomèdica, Universitat de Barcelona, Martí i Franquès 1, 08028 Barcelona, Catalonia; Institute of Nanoscience and Nanotechnology (IN2UB), Universitat de Barcelona, 08028 Barcelona, Catalonia, Spain.

Ultramicroscopy
|February 10, 2020
PubMed
Summary
This summary is machine-generated.

Fast and automated diffraction tomography (Fast-ADT) enables detailed analysis of nanocrystals using transmission electron microscopy. This technique enhances crystal structure determination and disorder identification for various materials.

More Related Videos

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

4.0K
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

11.3K

Related Experiment Videos

Last Updated: Dec 29, 2025

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging
08:55

Cryo-Electron Tomography Remote Data Collection and Subtomogram Averaging

Published on: July 12, 2022

5.7K
Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography
14:56

Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

Published on: May 20, 2022

4.0K
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
11:33

Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography

Published on: January 30, 2016

11.3K

Area of Science:

  • Materials Science
  • Crystallography
  • Electron Microscopy

Background:

  • Electron crystallography traditionally analyzes microcrystals, often organic compounds.
  • Transmission electron microscopy (TEM) offers nanoscale analytical capabilities.
  • Advancements in direct detection cameras and continuous stage tilting have spurred microcrystal analysis.

Purpose of the Study:

  • To introduce a new acquisition method, fast and automated diffraction tomography (Fast-ADT), for transmission electron microscopy.
  • To demonstrate the application of Fast-ADT for comprehensive diffraction data collection.
  • To validate Fast-ADT for structure determination, refinement, and disorder analysis.

Main Methods:

  • Development of Fast-ADT as a general application for TEM and STEM modes.
  • Implementation involving two sequential tilt scans: crystal tracking and diffraction tomography acquisition.
  • Application on various samples including BaSO4 crystals, RUB-5 layered silicate, and a DRED1 organic molecule polymorph.

Main Results:

  • Successful structure determination and refinement of BaSO4 crystals using Fast-ADT.
  • Demonstration of Fast-ADT's advantage in identifying subtle disorder details in RUB-5 layered silicate.
  • Ab initio structure solution and X-ray powder diffraction refinement of a new DRED1 organic molecule polymorph.

Conclusions:

  • Fast-ADT is a versatile technique applicable to any TEM, regardless of scanning unit availability.
  • The method effectively extracts maximum diffraction information from nanoscale crystalline materials.
  • Fast-ADT facilitates comprehensive structural analysis, including ab initio structure solution and disorder characterization.