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Aging is a complex biological phenomenon influenced by various processes that affect cellular and systemic functions. Several prominent theories attempt to explain its mechanisms, highlighting cellular limitations, oxidative damage, and hormonal changes as central factors in aging.
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Updated: Dec 29, 2025

Substructure Analyzer: A User-Friendly Workflow for Rapid Exploration and Accurate Analysis of Cellular Bodies in Fluorescence Microscopy Images
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Au nanozyme-driven antioxidation for preventing frailty.

Jeonghyo Kim1, Sangjin Oh1, Yong Cheol Shin2

  • 1Department of Chemistry, Chungnam National University, Daejeon, 34134, Republic of Korea.

Colloids and Surfaces. B, Biointerfaces
|February 10, 2020
PubMed
Summary
This summary is machine-generated.

Artificial nanoscale enzymes (nanozymes) composed of gold nanoparticles and phytochemicals show antioxidant and anti-aging effects. These nanozymes effectively prevent senescence and oxidative stress, suggesting potential for novel frailty therapeutics.

Keywords:
Anti-agingAu nanoparticleFrailtyNanozymePhytochemicalSenescence

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Gerontology

Background:

  • Cellular senescence and frailty arise from various biological processes.
  • Unregulated oxygen metabolism by-products cause cell damage.
  • The human body possesses antioxidant defense enzymes to mitigate oxidative stress.

Purpose of the Study:

  • To introduce artificial nanoscale enzymes (nanozymes) with antioxidant properties.
  • To evaluate the anti-aging and antioxidant effects of gold nanoparticle-based nanozymes (GI-Au NZs).
  • To investigate the therapeutic potential of nanozymes for senescence and frailty.

Main Methods:

  • Synthesis of gold nanoparticles (Au NPs) with gallic acid (GA) and isoflavone (IF) to create GI-Au NZs.
  • Assessment of antioxidant and anti-aging effects using Cell Counting Kit-8 and senescence-associated β-galactosidase assays on human dermal fibroblasts (nHDFs).
  • Evaluation of topical nanozyme application on mouse skin histology, SOD activity, and monitoring for hepatotoxicity and nephrotoxicity.

Main Results:

  • GI-Au NZs demonstrated significant antioxidant and anti-aging effects in nHDFs.
  • Topical application of NZs prevented oxidative stress and altered epidermal thickness in mice skin.
  • No significant hepatotoxicity or nephrotoxicity was observed in the treated mice.

Conclusions:

  • Nanozymes effectively prevent senescence in human cells and oxidative stress in mouse skin.
  • GI-Au NZs show promise as novel therapeutics for age-related frailty.
  • Further development of nanozyme-based strategies for acute frailty is suggested.