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Updated: Dec 29, 2025

Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Complete genome sequence of a novel putative polerovirus detected in grapevine
1Grape and Persimmon Research Station, Institute of Fruit Tree and Tea Science, National Agriculture and Food Research Organization (NARO), Akitsu, Higashihiroshima, Hiroshima, 739-2494, Japan. chiakiy151@affrc.go.jp.
Abstract:
Next-generation sequencing detected a novel virus from grapevine cultivar 'Kishmish Chjornyj' from Russia. Its complete genome sequence of 5625 nucleotides includes seven open reading frames encoding seven putative proteins similar to those of members of the genus Polerovirus in the family Luteoviridae. The novel virus showed graft-transmissibility and was tentatively named "grapevine polerovirus 1" (GPoV-1). Phylogenetic analysis using complete genome sequences of GPoV-1 and members of the family Luteoviridae indicated that although GPoV-1 is a member of the genus Polerovirus, it is unique within its clade. GPoV-1 is the first polerovirus detected in grapevine.
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