An All-in-One Aptasensor Integrating Enzyme Powered Three-Dimensional DNA Machine for Antibiotic Detection

Area of Science:

• Analytical Chemistry
• Biotechnology
• Food Science

Background:

• Traditional antibiotic detection methods are often time-consuming and complex.
• There is a need for rapid, sensitive, and accurate methods for antibiotic residue monitoring in food products.

Purpose of the Study:

• To develop an integrated aptasensor for efficient antibiotic detection.
• To enhance signal amplification for improved sensitivity and reduced detection time.

Main Methods:

• Development of an all-in-one aptasensor utilizing an enzyme-driven 3D DNA walker.
• Anchoring high-density substrate strands on the walking interface to accelerate signal amplification.
• Kanamycin detection via fluorescence enhancement triggered by DNA walker activity.

Main Results:

• The aptasensor achieved a low detection limit of 1.23 pM for kanamycin.
• The entire detection process was completed within 40 minutes.
• Satisfactory recoveries (97.76%–105.33%) were obtained in spiked milk samples, indicating high accuracy and stability.

Conclusions:

• The developed all-in-one aptasensor provides a rapid and sensitive platform for antibiotic detection.
• This technology holds significant potential for ensuring food safety and quality control.