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Atropine and DFP-induced delayed neurotoxicity.
T P Kellner1, J D Henderson, R J Higgins
1Department of Avian Sciences and Environmental Toxicology, University of California, Davis 95616.
Neurotoxicology
|January 1, 1988
Summary
Atropine, an antidote for organophosphate poisoning, delayed the onset of organophosphate-induced delayed neuropathy (OPIDN) in chickens. This study investigated atropine
Area of Science:
- Neuroscience
- Toxicology
- Pharmacology
Background:
- Organophosphorus esters can cause organophosphate-induced delayed neuropathy (OPIDN).
- Atropine is a common antidote for acute cholinergic effects.
- The effect of atropine on OPIDN onset and severity is not fully understood.
Purpose of the Study:
- To investigate the impact of atropine on the onset and severity of OPIDN.
- To determine if atropine influences neurotoxic esterase (NTE) activity in vivo and in vitro.
Main Methods:
- Chickens were administered diisopropyl phosphorofluoridate (DFP) with or without atropine.
- Locomotion, brain neurotoxic esterase (NTE) activity, and nervous system histology were assessed.
- In vitro assays were performed to evaluate atropine's inhibition of NTE.
Main Results:
- Atropine treatment delayed the onset of OPIDN signs in chickens.
- A slight increase in brain NTE activity was observed in vivo following atropine administration.
- Atropine demonstrated NTE inhibitory activity in vitro at high concentrations (Ki: ~3.0 mM).
Conclusions:
- Atropine may offer a protective effect against the development of OPIDN.
- The findings suggest a complex interaction between atropine and the mechanisms underlying OPIDN.
- Further research is warranted to elucidate atropine's role in mitigating organophosphate-induced neurotoxicity.