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Updated: Dec 28, 2025

Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA
Published on: June 25, 2014
Data on multimerization efficiency for short linear DNA templates and phosphoryl guanidine primers during isothermal
Ravil R Garafutdinov1, Assol R Sakhabutdinova1, Maxim S Kupryushkin2
1Institute of Biochemistry and Genetics, Ufa Federal Research Center of the Russian Academy of Sciences, Russian Federation.
Abstract:
This article reports experimental data related to the research article entitled "Prevention of DNA multimerization using phosphoryl guanidine primers during isothermal amplification with Bst exo- DNA polymerase" (R.R. Garafutdinov, A.R. Sakhabutdinova, M.S. Kupryushkin, D.V. Pyshnyi, 2020) [1]. Here, multimerization efficiency in terms of Tt (time-to-threshold) values obtained for artificial DNA templates with the different nucleotide sequences during isothermal amplification with Bst exo- DNA polymerase is given. Data on the influence of phosphoryl guanidine primers (PGO) on multimerization for the LTc template which has shown high efficiency of multimerization are presented as well.
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