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FISH for Pre-implantation Genetic Diagnosis
Published on: February 23, 2011
Porcine malignant hyperthermia carrier detection and chromosomal assignment using a linked probe
1Department of Biochemistry, Norwegian College of Veterinary Medicine, Oslo.
Animal Genetics
|January 1, 1988
Summary
A new DNA probe (GPI8R) helps detect genetic variations linked to malignant hyperthermia (MH) in pigs. This discovery aids in identifying carriers of the halothane (HAL) gene, improving animal health management.
Area of Science:
- Genetics
- Animal Breeding
- Molecular Biology
Background:
- The glucosephosphate isomerase (GPI) gene in pigs is closely associated with the halothane (HAL) gene.
- The HAL gene is responsible for malignant hyperthermia (MH), a serious condition in susceptible pigs.
Purpose of the Study:
- To develop a DNA probe for detecting polymorphisms in the GPI gene.
- To confirm the linkage between the GPI gene polymorphism and the HAL locus.
- To determine the chromosomal location of the GPI gene.
Main Methods:
- Isolation of a single-copy DNA probe (GPI8R) from a pig genomic library.
- Analysis of pig DNA polymorphisms using SacI and PvuII restriction enzymes.
- Family studies to establish genetic linkage.
- In situ hybridization to map the GPI gene locus.
Main Results:
- The GPI8R probe identified a five-allele polymorphism in pig DNA.
- This GPI polymorphism was shown to be linked to the HAL locus.
- In situ hybridization mapped the GPI gene to chromosome 6 (bands p12-q22).
Conclusions:
- The GPI8R probe is a valuable tool for carrier detection of the HAL gene mutation.
- The HAL linkage group, including the GPI and HAL genes, is located on pig chromosome 6.
