Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.1K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.1K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

2.4K
2.4K
Transcriptional Regulation: Riboswitches01:23

Transcriptional Regulation: Riboswitches

458
Riboswitches are RNA elements that regulate gene expression by altering their secondary structures in response to specific effector molecules. These elements, located in the leader regions of certain mRNAs, act as transcriptional regulators by toggling between alternative conformations to control downstream gene expression. Riboswitch-mediated regulation is a precise mechanism for modulating biosynthetic pathways, as exemplified by the riboflavin biosynthesis pathway in Bacillus...
458
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

11.4K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.4K
Translational Regulation01:29

Translational Regulation

460
Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
460
Riboswitches01:56

Riboswitches

9.4K
Riboswitches are non-coding mRNA domains that regulate the transcription and translation of downstream genes without the help of proteins. Riboswitches bind directly to a metabolite and can form unique stem-loop or hairpin structures in response to the amount of the metabolite present. They have two distinct regions – a metabolite-binding aptamer and an expression platform.
The aptamer has high specificity for a particular metabolite which allows riboswitches to specifically regulate...
9.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Real-time imaging of transcriptional feedback in nonsense-mediated mRNA decay.

Science advances·2026
Same author

Spatially defined microenvironmental niches are associated with clinical outcome and tumor ecosystem diversity in head and neck cancer.

Med (New York, N.Y.)·2026
Same author

Outcomes of Adolescents and Young Adults with AML Treated on Pediatric vs Adult Protocols.

Blood advances·2026
Same author

An integrated RNA-centric imaging and omics approach reveals distinct properties and composition of neuronal RNA granules.

bioRxiv : the preprint server for biology·2026
Same author

RNA-binding proteins in the mouse lens: Functional classifications, expression profiling, and interaction studies of Carhsp1 with crystallin mRNAs.

Developmental biology·2026
Same author

A series of spontaneously blinking dyes for super-resolution microscopy.

Nature methods·2026
Same journal

Functional and Mechanistic Interplay of Host and Viral Alternative Splicing Regulation during Influenza Infection.

Cold Spring Harbor symposia on quantitative biology·2020
Same journal

Myriad RNAs and RNA-Binding Proteins Control Cell Functions, Explain Diseases, and Guide New Therapies.

Cold Spring Harbor symposia on quantitative biology·2020
Same journal

Small RNA Function in Plants: From Chromatin to the Next Generation.

Cold Spring Harbor symposia on quantitative biology·2020
Same journal

U1 snRNP Telescripting Roles in Transcription and Its Mechanism.

Cold Spring Harbor symposia on quantitative biology·2020
Same journal

The THO Complex as a Paradigm for the Prevention of Cotranscriptional R-Loops.

Cold Spring Harbor symposia on quantitative biology·2020
Same journal

Biophysical Properties of HP1-Mediated Heterochromatin.

Cold Spring Harbor symposia on quantitative biology·2020
See all related articles

Related Experiment Video

Updated: Dec 28, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

7.0K

Zipcode Binding Protein 1 (ZBP1; IGF2BP1): A Model for Sequence-Specific RNA Regulation.

Jeetayu Biswas1, Leti Nunez1, Sulagna Das1

  • 1Department of Anatomy and Structural Biology, Albert Einstein College of Medicine, Bronx, New York 10461, USA.

Cold Spring Harbor Symposia on Quantitative Biology
|February 23, 2020
PubMed
Summary
This summary is machine-generated.

RNA binding proteins (RBPs) control RNA fate. This review highlights how ZBP1 (IGF2BP1) binding to β-actin mRNA exemplifies RBP-mediated RNA localization and translation regulation.

More Related Videos

An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.9K
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

9.4K

Related Experiment Videos

Last Updated: Dec 28, 2025

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins
11:34

Exploring Sequence Space to Identify Binding Sites for Regulatory RNA-Binding Proteins

Published on: August 9, 2019

7.0K
An Assay for Quantifying Protein-RNA Binding in Bacteria
07:02

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

6.9K
Novel RNA-Binding Proteins Isolation by the RaPID Methodology
11:19

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

9.4K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Biochemistry

Background:

  • RNA binding proteins (RBPs) are crucial regulators of RNA metabolism, influencing localization, translation, and decay.
  • β-actin mRNA serves as a model system for studying RNA localization and the role of RBPs in eukaryotic cells.
  • Z-binding protein 1 (ZBP1), also known as IGF2BP1, is a key RBP that controls β-actin mRNA localization and translation.

Purpose of the Study:

  • To summarize decades of research on β-actin mRNA and its interactions with RBPs.
  • To highlight ZBP1 as a model protein for understanding RBP-mRNA interactions.
  • To discuss the impact of technological advancements on the study of RNA-RBP dynamics.

Main Methods:

  • Review of published literature on β-actin mRNA and ZBP1.
  • Analysis of experimental data detailing RNA localization and translation control.
  • Examination of technological innovations in RNA and RBP research.

Main Results:

  • ZBP1 binding to β-actin mRNA is critical for its proper localization and translational control.
  • Studies on β-actin mRNA have elucidated fundamental mechanisms of RBP-mediated gene regulation.
  • Technological progress has significantly enhanced the ability to study RBP-RNA interactions in vivo and in vitro.

Conclusions:

  • ZBP1 is a paradigm for understanding how RBPs regulate mRNA fate.
  • Continued technological innovation is essential for addressing remaining questions in RBP-mediated mRNA regulation.
  • Understanding RBP-mRNA interactions is key to deciphering cellular processes and disease mechanisms.