Related Experiment Video

Updated: Dec 27, 2025

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS
14:15

Preparation of Myeloid Derived Suppressor Cells MDSC from Naive and Pancreatic Tumor-bearing Mice using Flow Cytometry and Automated Magnetic Activated Cell Sorting AutoMACS

Published on: June 18, 2012

26.7K

Myeloid derived suppressor cells contribute to the malignant progression of oral squamous cell carcinoma

Xin Pang1, Hua-Yang Fan1, Ya-Ling Tang1

  • 1State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.

Plos One
|February 25, 2020
PubMed
Abstract

Insights

Myeloid-derived suppressor cells (MDSCs) fuel oral squamous cell carcinoma (OSCC) progression by promoting proliferation, invasion, and immune suppression. Targeting this crosstalk offers new therapeutic avenues for OSCC treatment.

Area of Science:

  • Oncology
  • Immunology
  • Oral Pathology

Background:

  • Myeloid-derived suppressor cells (MDSCs) are key immunosuppressive cells within the tumor microenvironment.
  • MDSCs significantly influence cancer progression and patient prognosis.

Purpose of the Study:

  • To investigate the interaction between MDSCs and oral squamous cell carcinoma (OSCC) cells.
  • To elucidate the role of this crosstalk in OSCC malignant progression and immune suppression.

Main Methods:

  • Immunohistochemistry (IHC) for CD33 expression in OSCC tissues.
  • Magnetic-activated cell sorting (MACS) for MDSC enrichment and flow cytometry for phenotype identification.
  • Co-culture systems of MDSCs and OSCC cells to assess proliferation, apoptosis, migration, invasion, epithelial-mesenchymal transition (EMT), and vasculogenic mimicry (VM).

Main Results:

  • Increased MDSC numbers in OSCC tissues correlated with advanced tumor stage, grade, metastasis, and poor prognosis.
  • MDSCs enhanced OSCC proliferation, migration, invasion, EMT, and VM formation in co-culture models.
  • OSCC cells induced MDSC differentiation from peripheral blood mononuclear cells (PBMCs) and increased Arginase-1 (Arg-1) and inducible nitric oxide synthase (iNOS) expression.

Conclusions:

  • The crosstalk between MDSCs and OSCC cells promotes tumor malignancy and immune suppression.
  • Targeting tumor-associated immunosuppressive cells like MDSCs presents a promising therapeutic strategy for OSCC.

More Related Videos

Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics11:28

Isolation and Characterization of a Head and Neck Squamous Cell Carcinoma Subpopulation Having Stem Cell Characteristics

Published on: May 11, 2016

8.9K
Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies06:33

Author Spotlight: Analyzing Bone Marrow Microenvironment in Murine Hematological Malignancies

Published on: November 10, 2023

1.7K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.0K
Differentiation of Common Myeloid Progenitor Cells01:15

Differentiation of Common Myeloid Progenitor Cells

Common myeloid progenitors (CMPs) are oligopotent cells that can differentiate into granulocytes and macrophages. Granulocytes and macrophages are essential for protecting the body against bacterial, viral, or fungal infections. They migrate from the bone marrow into the circulating blood to reach specific tissue sites where they differentiate and help in immune surveillance. However, they survive only for a few days and must be continuously made available to the organism to maintain a robust...
3.8K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.8K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
5.4K
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
7.1K
Cancer-Critical Genes II: Tumor Suppressor Genes01:05

Cancer-Critical Genes II: Tumor Suppressor Genes

Genes usually encode proteins necessary for the proper functioning of a healthy cell. Mutations can often cause changes to the gene expression pattern, thereby altering the phenotype.
When the function of certain critical genes, especially those involved in cell cycle regulation and cell growth signaling cascades, gets disrupted, it upsets the cell cycle progression. Such cells with unchecked cell cycles start proliferating uncontrollably and eventually develop into tumors.
Such genes that act...
9.3K