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Updated: Dec 27, 2025
10:24
Next-generation Sequencing of 16S Ribosomal RNA Gene Amplicons
Published on: August 29, 2014
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Mothulity Facilitates 16S/ITS Amplicon Diversity Analysis
D Izak1, A Gromadka1, S Kaczanowski1
1Department of Bioinformatics, Institute of Biochemistry and Biophysics Polish Academy of Science, Warsaw, Poland.
Current Protocols in Bioinformatics
|February 25, 2020
Summary
Mothulity simplifies microbial community analysis by providing an intuitive interface for the Mothur software. This tool reduces errors and speeds up the process, generating high-quality figures for biodiversity studies.
Area of Science:
- Microbiology
- Bioinformatics
- Computational Biology
Background:
- Mothur is a widely used software for microbial community analysis (16S/ITS sequencing).
- Executing Mothur can be complex for new users and time-consuming for experienced users.
- Mothur lacks graphical output, requiring users to generate plots separately.
Purpose of the Study:
- Introduce Mothulity, a novel graphical user interface for Mothur.
- To streamline and simplify the 16S/ITS biodiversity analysis workflow.
- To reduce human error and accelerate analysis time.
Main Methods:
- Developed a minimalistic yet powerful interface for Mothur.
- Automated and predefined most analysis parameters.
- Integrated SLURM workload manager compatibility.
Main Results:
- Mothulity minimizes human error in Mothur analysis.
- Significantly reduces the time required for biodiversity analysis.
- Generates an HTML report with publication-quality figures.
Conclusions:
- Mothulity enhances the usability of Mothur for 16S/ITS biodiversity analysis.
- The tool is suitable for various computing environments, including those using SLURM.
- Facilitates faster and more reliable microbial community analysis.
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